duoset elisa development systems  (R&D Systems)

Journal: Parasite Immunology

Article Title: The Regulatory Effect of Histone Deacetylase ( HDAC ) 1 and 2 on iNOS , IL ‐6, TNF ‐α and IL ‐10 Expression in Canine Macrophages Infected With Leishmania infantum

doi: 10.1111/pim.70078

Figure Lengend Snippet: Inhibition of HDAC1 in cells infected with Leishmania infantum and treated with sodium butyrate on IL‐6, TNF‐α and IL‐10 expression. DH82 cells were treated with the pharmacological HDAC1 inhibitor sodium butyrate NaB (10 mM) and (20 mM). After 6 h of infection, the culture supernatant cells were harvested, and (A) IL‐6, (B) TNF‐α and (C) IL‐10 were measured by capture ELISA. The statistical test used was the Friedman test, followed by Dunn's multiple‐comparison test ( p < 0.05).

Article Snippet: Cytokines IL‐6, TNF‐α and IL‐10 were quantified in culture supernatant from L. infantum infected macrophages after HDAC1 and HDAC2 suppression with NaB (10 mM) and (20 mM) (Sigma‐Aldrich, MO, USA) by using the DuoSet ELISA Development Systems canine kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's instructions.

Techniques: Inhibition, Infection, Expressing, Enzyme-linked Immunosorbent Assay, Comparison